Salmonella serotyping by the coagglutination method using protein A rich Staphylococcus sensitized with specific antibody.
نویسندگان
چکیده
This paper outlines a more economical method in the serological identification of salmonella by using protein-A rich staphylococci sensitized with Salmonella polyvalent 0 and H antisera. Preliminary studies show that this method is simple, economical and as sensitive and specific as the original commercially prepared antisera with the exception of Salmonella typhi where there appears to be some loss of sensitivity with these strains.
منابع مشابه
Method for identifying Salmonella and Shigella directly from the primary isolation plate by coagglutination of protein A-containing staphylococci sensitized with specific antibody.
A technique is described that allows presumptive identification of either Salmonella or Shigella organisms directly upon the original isolation plate, in this case, MacConkey agar. This was accomplished by applying a drop of specifically sensitized protein A-containing Staphylococcus aureus over a "suspected" colony or several colonies of organisms grown on MacConkey agar. The plate is tilted t...
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In our investigation, rabbit hyper-immune serum to V.cholerae ogawa was absorbed with V.cholerae inaba whole-cells and vice versa. Applying ammonium sulphate precipitation method, mono-specific g globulins were purified and concentrated from the absorbed whole serum. These antibodies were fixed on staphylococcus cowan 1 NCTC-8325 whole-cells, using different chemical fixatives. It was observed ...
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ورودعنوان ژورنال:
- The Malaysian journal of pathology
دوره 2 شماره
صفحات -
تاریخ انتشار 1979